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If that floor is pretty stable you might be able to just put shluter right on the pine and save some height.
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No way.
You can not install Ditra, or any other membrane or concrete backer board over plank flooring. You need a plywood underlayment first. If those 2x4's are perfectly flat you can go with 1/2" B/C exposure 1 ply, otherwise you will need 5/8 or 3/4".
Tile is not gonna make the floor waterproof, although bathroom floors are not usually waterproofed.
Make a wood or marble threshold transition in the doorway.
Jaz