winsox, the most likely sources are that a decking board has popped loose or you have shingles that were improperly installed. If the shingles are still intack, you could carefully lift the tab, install a new fastener, and hand seal the shingle back together using roof cement. If it is a decking issue you may have to remove the shingles in that area, find the rafter and re-attach the decking with I'd suggest decking screws. Then you would need to install new shingles.
Oh, and welcome to House Repair Talk.